| Please fill out this form once for each virus request. |
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Customer ID:
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Your name: |
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Your email address:
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Name of PI:
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| How many viruses are in this set? |
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| Your PO number(s): |
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| Is your gene of interest already in a viral vector plasmid that can be used to generate virus by transfection? |
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| If the answer is yes, please proceed to Section 2. |
Section 1.
If the gene of interest is not in a viral vector, we can help you to subclone it into the desired viral vector. You will need to provide us a plasmid as the source of the gene and also relevant information for subcloning.
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Which virus do you want the gene inserted into? |
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| Do you want GFP expression in addition to your gene? |
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| Do you want any special expression components? |
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- If so, then please describe them:
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| If you are not certain about any of these questions, please contact Dr. Zhanyun Fan (via Email).
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About the plasmid that you are submitting to us as the source of your gene of interest:
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| What is the name of the plasmid? |
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| Who made the plasmid? |
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| If you have the complete sequence of the plasmid, please copy it as plain text and paste it here:
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| Please tell us here the exact numeric coordinates in your sequence file corresponding to the start and stop codons of the open reading frame that you intend to move into the vector. |
Start codon:
Stop codon: |
| In case you do not have access to the complete sequence of the entire plasmid, we would at least need the sequence of your gene of interest as well as the flanking restriction enzyme sites that can potentially be used for subcloning. Please copy the sequence as plain text and paste it here: |
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| Please tell us here the exact numeric coordinates in your sequence file corresponding to the start and stop codons of the open reading frame that you intend to move into the vector. |
Start codon:
Stop codon: |
| If your gene sequence is in Genbank, please enter its accession number: |
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Section 2.
Your gene of interest is already in a viral vector plasmid. |
| If you have contacted us before and know that the plasmid is in the HGTI plasmid collection, then please provide the name of the plasmid: |
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If you are submitting your own vector plasmid: |
| What is the name of the plasmid? | |
| Who made the plasmid? | |
| If you have the complete sequence of the plasmid, please copy it as plain text and paste it here: |
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| Please tell us here the exact numeric coordinates in your sequence file corresponding to the start and stop codons of your gene of interest. | Start codon:
Stop codon: |
| In case you do not have access to the complete sequence of the entire plasmid, please provide any information about the plasmid that you think would be relevant to virus production. If you have sequencing data on a segment of the plasmid, please copy it as plain text and paste it here. |
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| If your gene sequence is in Genbank, please enter its accession number: |
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Section 3.
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| Is there a potential that this gene could be oncogenic? |
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Have you obtained institutional approval for working with recombinant viruses in your facility? |
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Have you obtained institutional approval for working with the specific viruses you wish us to make for you?
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And finally, please provide a summary of your research project (1-2 paragraphs) and why the use of the new viruses will help you address specific biological questions. A model example of this is given below: Failure to provide a complete abstract will prevent the processing of your request.
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PROJECT DESCRIPTION (sample)
BACKGROUND:
Recent studies suggest that the xyz gene is important in cell differentiation. This activity is restricted to myeloid cells, and the mutations in this gene are consistently found in cells derived from chronic myeloid leukemia (CML) patients.
EXPERIMENTAL APPROACH AND GOALS:
We are requesting two retroviral vectors expressing the xyz gene in its sense and antisense orientation to carry out experiments that have the following objectives:
- 1. to evaluate whether the transduction of the wild type xyz gene corrects the defect of CML cells; and
- 2. to evaluate whether transduction of the antisense xyz gene in normal myeloid cells affects their differentiation ability.
LONG TERM GOALS:
The long term goal of the project is the development of gene therapy methods for the treatment of CML.
(This abstract is fabricated and the science described herein is false; it is an example of a model abstract.)
Failure to provide a complete abstract will prevent the processing of your request.
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BACKGROUND:
EXPERIMENTAL APPROACH AND GOALS:
LONG TERM GOALS:
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