Plasmid Name = MMP Database Index Number = 1648 Created by: JSL Description: FEATURES Location/Qualifiers intron 1202..2142 /label="MoMLV_intron" /note="Intron from MoMLV, as it appears in Mulligan lab MMP and MFG-S constructions." repeat_region 997..1064 /label="R_MPSV" /note="R region from MMP, which is originally derived from MPSV." repeat_region 3029..3096 /label="R_MPSV" /note="R region from MMP, which is originally derived from MPSV." repeat_region 1065..1138 /label="U5_MPSV" /note="Erroneous U5 from MPSV. Error made when copying bad sequence information from the 3 prime U5. Present in almost all MMP constructs." repeat_region 3097..3170 /label="U5_MPSV" /note="Erroneous U5 from MPSV. Error made when copying bad sequence information from the 3 prime U5. Present in almost all MMP constructs." repeat_region 552..996 /label="U3_MPSV" /note="U3 from MMP, which contains elements from MPSV." repeat_region 2584..3028 /label="U3_MPSV" /note="U3 from MMP, which contains elements from MPSV." misc_feature 1617..1634 /label="MFGS_gag" /note="gag ATG, as it appears in MFGS constructs." misc_feature 2582..2594 /label="MLV_U3_tip" /note="Tip of both MLV and MPSV U3. Begins the U3 region." misc_feature 1407..1424 /label="MFGS_csStop" /note="Engineered stop codon from MFG-S, SFG, and MMP vector constructs. Terminates the cell surface gag orf 18 amino acids after the CTG start codon." misc_feature 1353..1616 /label="MFGS_CSgag" /note="Mutated form of the Moloney Cell Surface gag extension. Starting at the CTG, this sequence is translated into the gag open reading frame, in order to make the cell surface form of the gag polyprotein xxglycogagxx. Here there are two mutations, one which introduces a stop codon after the 18th codon, and another which is compensatory for maintaining the secondary structure of the packaging signal in this region." CDS complement(4863..5720) /label="bla" /note="B-lactamase, confers ampicillin resistance, no Pst I." misc_feature complement(3723..3739) /label="lacO" /note="lac operator; bound by LacI repressor to repress lac operon expression" misc_feature complement(4521..4564) /label="Low_copy" /note="Tag for low copy plasmid replication xxpBR322xx" misc_feature complement(1128..1140) /label="MLV_U3_tip" /note="Tip of both MLV and MPSV U3. Begins the U3 region." misc_feature complement(1141..1158) /label="PBS-Q" /note="tRNA binding site for MMP vectors, for Glutamine tRNA." CDS complement(6156..6314) /label="lacZ_alphaxx" /note="Fragment of lacZ alpha sequence that is used in pUC. Should also pick up the fragment in other plasmids." misc_feature 4526..4557 /label="Correction-190" /note="Difference between low and high copy plasmids. Many of our lab plasmids are high copy, yet the sequence file suggests that they are low copy. This correction could be applied to make the sequence properly represent the actual DNA sequence for a high copy plasmid. It was confirmed in the full plasmid sequencing of CMMP-MR1-1TcR, which has the pUC18 backbone. This sequence has been found to be erroneous for some plasmids in our laboratory. It would be prudent to sequence the DNA to confirm the accuracy of your records. The sequence specified by this feature was actually found to be: ACGGCTACAC TAGAAGAACA GTATTTGGTA TC. " misc_feature 3150..3194 /label="Correction-194" /note="From complete sequence of MR1-1 TcR fusion CMMP vector. Shows error in 3 prime U5. Correct sequence is actually identical to MLV in this region. This sequence has been found to be erroneous for some plasmids in our laboratory. It would be prudent to sequence the DNA to confirm the accuracy of your records. The sequence specified by this feature was actually found to be: ACCCGTCAGC GGGGGTCTTT CAGTTTCTCC CACCTACACA GGTCTC. " misc_feature complement(3690..3713) /label="Correction-189" /note="pUC18 instability. This deletion has been shown to occur in many plasmids based upon pUC18 and similar plasmids. It involves a deletion of the cytosine nucleotide from the second codon of the lacZ alpha gene, just upstream of the polylinker. Some constructs in our lab have the deletion, and some do not. There is no apparent restriction enzyme polymorphism, but the deletion can affect the performance of some sequencing primers. See Lobet, Y. et al., 1989, NAR v17, 4897. This sequence has been found to be erroneous for some plasmids in our laboratory. It would be prudent to sequence the DNA to confirm the accuracy of your records. The sequence specified by this feature was actually found to be: GGAAACAGCT ATGACATGAT TAC. " CDS 1720..1744 /label="HGTI-1043_Max=0" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 0 bases. Perfect match Tm = 61.1 C, although only 17 nt are required to match. The actual sequence of the primer is GACGTTGGGT TACCTTCTGC TCTGC." CDS 1920..1948 /label="HGTI-1044_Max=561" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 561 bases. Perfect match Tm = 67.0 C, although only 17 nt are required to match. The actual sequence of the primer is TTTGTACACC CTAAGCCTCC GCCTCCTCT." CDS 2073..2102 /label="HGTI-1065_Max=679" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 679 bases. Perfect match Tm = 73.6 C, although only 17 nt are required to match. The actual sequence of the primer is GCACCCCCGC CCCTTGTAAA CTTCCCTGAC ." CDS 2266..2291 /label="HGTI-1066_Max=663" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 663 bases. Perfect match Tm = 62.8 C, although only 17 nt are required to match. The actual sequence of the primer is TCCGCCGACA CCAGACTAAG AACCTA." CDS complement(1659..1687) /label="HGTI-1067_Max=524" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 524 bases. Perfect match Tm = 65.4 C, although only 17 nt are required to match. The actual sequence of the primer is ATCCGCTCGA CATCTTTCCA GTGACCTAA." CDS complement(1864..1891) /label="HGTI-1068_Max=458" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 458 bases. Perfect match Tm = 69.3 C, although only 17 nt are required to match. The actual sequence of the primer is GCCAAGGCTT CCCAGGTCAC GATGTAGG." CDS complement(2012..2037) /label="HGTI-1069_Max=464" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 464 bases. Perfect match Tm = 57.5 C, although only 17 nt are required to match. The actual sequence of the primer is CTAGAGAAGG AGTGAGGGCT GGATAA." CDS complement(2205..2226) /label="HGTI-1090_Max=447" /note="Sequencing primers for MLV gagpol. Max recorded sequencing read = 447 bases. Perfect match Tm = 60.2 C, although only 17 nt are required to match. The actual sequence of the primer is CCGGTCGGTC CAGTTGTTCT TG." CDS complement(6182..6207) /label="HGTI-1288_Max=" /note="sequencing primers for AAV backbone Max recorded sequencing read = bases. Perfect match Tm = 72.8 C, although only 17 nt are required to match. The actual sequence of the primer is CCGCACCGAT CGCCCTTCCC AACAGT." CDS 6030..6053 /label="HGTI-1291_Max=577" /note="sequencing primers for AAV backbone Max recorded sequencing read = 577 bases. Perfect match Tm = 71.4 C, although only 17 nt are required to match. The actual sequence of the primer is GTCAGCGGGT GTTGGCGGGT GTCG." CDS 2345..2368 /label="HGTI-1292_Max=563" /note="retrovirus sequencing primer forward Max recorded sequencing read = 563 bases. Perfect match Tm = 61.8 C, although only 17 nt are required to match. The actual sequence of the primer is caaagtagac ggcatcgcag cttg." CDS complement(2484..2509) /label="HGTI-1293_Max=467" /note="sequencing primer for retrovirus reverse Max recorded sequencing read = 467 bases. Perfect match Tm = 51.1 C, although only 17 nt are required to match. The actual sequence of the primer is tgatattgtt gagtcaaaac tagagc." CDS 1139..1168 /label="HGTI-1604_Max=" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = bases. Perfect match Tm = 70.1 C, although only 17 nt are required to match. The actual sequence of the primer is TTTGGAGGTT CCACCGAGAT TTGGAGACCC ." CDS 1389..1418 /label="HGTI-1605_Max=446" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 446 bases. Perfect match Tm = 70.6 C, although only 17 nt are required to match. The actual sequence of the primer is GTGGCCCGAC CTGAGTCCTA AAATCCCGAT ." CDS 1639..1668 /label="HGTI-1606_Max=559" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 559 bases. Perfect match Tm = 61.3 C, although only 17 nt are required to match. The actual sequence of the primer is CCACTCCCTT AAGTTTGACC TTAGGTCACT ." CDS 1889..1918 /label="HGTI-1607_Max=163" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 163 bases. Perfect match Tm = 75.7 C, although only 17 nt are required to match. The actual sequence of the primer is GGCTTTTGAC CCCCCTCCCT GGGTCAAGCC ." CDS 2139..2168 /label="HGTI-1608_Max=716" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 716 bases. Perfect match Tm = 57.3 C, although only 17 nt are required to match. The actual sequence of the primer is CAAGCTCACT TACAGGCTCT CTACTTAGTC ." CDS 2389..2418 /label="HGTI-1609_Max=0" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 0 bases. Perfect match Tm = 80.3 C, although only 17 nt are required to match. The actual sequence of the primer is AGGCTGCCGA CCCCGGGGGT GGACCATCCT ." CDS 2528..2557 /label="HGTI-1627_Max=509" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 509 bases. Perfect match Tm = 54.4 C, although only 17 nt are required to match. The actual sequence of the primer is GTACGAGCCA TAGATAAAAT AAAAGATTTT ." CDS complement(2554..2583) /label="HGTI-1628_Max=" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = bases. Perfect match Tm = 62.2 C, although only 17 nt are required to match. The actual sequence of the primer is ttcccccctt tttctggaga ctaaataaaa ." CDS complement(2415..2444) /label="HGTI-1646_Max=" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = bases. Perfect match Tm = 70.6 C, although only 17 nt are required to match. The actual sequence of the primer is cccacgagtc ggacatggca gtctagagga ." CDS complement(2165..2194) /label="HGTI-1647_Max=607" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 607 bases. Perfect match Tm = 69.6 C, although only 17 nt are required to match. The actual sequence of the primer is gccagaggtc tccagacttc gtgctggact ." CDS complement(1915..1944) /label="HGTI-1648_Max=" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = bases. Perfect match Tm = 70.1 C, although only 17 nt are required to match. The actual sequence of the primer is gaggcggagg cttagggtgt acaaagggct ." CDS complement(1665..1694) /label="HGTI-1649_Max=" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = bases. Perfect match Tm = 71 C, although only 17 nt are required to match. The actual sequence of the primer is gtgagcgatc cgctcgacat ctttccagtg ." CDS complement(1415..1444) /label="HGTI-1650_Max=130" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = 130 bases. Perfect match Tm = 70.3 C, although only 17 nt are required to match. The actual sequence of the primer is gggggtgcac caaagagtcc taaacgatcg ." CDS complement(1165..1194) /label="HGTI-1651_Max=" /note="Sequencing primers for MMP-FAA, from LTR to LTR Max recorded sequencing read = bases. Perfect match Tm = 82.9 C, although only 17 nt are required to match. The actual sequence of the primer is cgggggggtc ggtggtccct gggcaggggt ." CDS 6059..6089 /label="HGTI-1662_Max=680" /note="Series of primers for construction of 4 variations of Adenovirus helper constructs, with potential for deletions in Psi. Max recorded sequencing read = 680 bases. Perfect match Tm = 67.2 C, although only 17 nt are required to match. The actual sequence of the primer is GGCTTAACTA TGCGGCATCA GAGCAGATTG T." CDS complement(6297..6319) /label="HGTI-1762_Max=" /note="Sequencing primer in lacZ, at 5xx end and facing upstream. Max recorded sequencing read = bases. Perfect match Tm = 57.4 C, although only 17 nt are required to match. The actual sequence of the primer is attcactggc cgtcgtttta caa." CDS complement(3281..3304) /label="HGTI-1766_Max=" /note="Sequencing primer downstream of MMP right LTR and facing left. Max recorded sequencing read = bases. Perfect match Tm = 54.8 C, although only 17 nt are required to match. The actual sequence of the primer is aaagccgaga gtcaggatgt agtc." CDS complement(3699..3720) /label="HGTI-1805_Max=673" /note="std sequencing primer -47 Max recorded sequencing read = 673 bases. Perfect match Tm = 49.4 C, although only 17 nt are required to match. The actual sequence of the primer is tcacacagga aacagctatg ac." CDS 4077..4096 /label="HGTI-1850_Max=589" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 589 bases. Perfect match Tm = 59.7 C, although only 17 nt are required to match. The actual sequence of the primer is taaaaaggcc gcgttgctgg ." CDS 4377..4395 /label="HGTI-1851_Max=601" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 601 bases. Perfect match Tm = 60.1 C, although only 17 nt are required to match. The actual sequence of the primer is cgaccgctgc gccttatcc." CDS 4677..4706 /label="HGTI-1852_Max=372" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 372 bases. Perfect match Tm = 58.8 C, although only 17 nt are required to match. The actual sequence of the primer is aaaaggatct caagaagatc ctttgatctt ." CDS 4977..4998 /label="HGTI-1853_Max=0" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 0 bases. Perfect match Tm = 61.0 C, although only 17 nt are required to match. The actual sequence of the primer is ccccagtgct gcaatgatac cg." CDS 5277..5297 /label="HGTI-1854_Max=650" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 650 bases. Perfect match Tm = 58.4 C, although only 17 nt are required to match. The actual sequence of the primer is agcggttagc tccttcggtc c." CDS 5577..5600 /label="HGTI-1855_Max=661" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 661 bases. Perfect match Tm = 58.0 C, although only 17 nt are required to match. The actual sequence of the primer is atccagttcg atgtaaccca ctcg." CDS 5877..5907 /label="HGTI-1856_Max=711" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 711 bases. Perfect match Tm = 58.3 C, although only 17 nt are required to match. The actual sequence of the primer is gacattaacc tataaaaata ggcgtatcac g." CDS complement(3864..3886) /label="HGTI-1880_Max=690" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 690 bases. Perfect match Tm = 59.4 C, although only 17 nt are required to match. The actual sequence of the primer is gttggccgat tcattaatgc agc." CDS complement(4161..4186) /label="HGTI-1881_Max=511" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 511 bases. Perfect match Tm = 59 C, although only 17 nt are required to match. The actual sequence of the primer is atctttatag tcctgtcggg tttcgc." CDS complement(4459..4486) /label="HGTI-1882_Max=565" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 565 bases. Perfect match Tm = 60.5 C, although only 17 nt are required to match. The actual sequence of the primer is catacctcgc tctgctaatc ctgttacc." CDS complement(4758..4786) /label="HGTI-1883_Max=564" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 564 bases. Perfect match Tm = 58.2 C, although only 17 nt are required to match. The actual sequence of the primer is tgaagatcct ttttgataat ctcatgacc." CDS complement(5062..5086) /label="HGTI-1884_Max=593" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 593 bases. Perfect match Tm = 59.6 C, although only 17 nt are required to match. The actual sequence of the primer is ataaagttgc aggaccactt ctgcg." CDS complement(5359..5386) /label="HGTI-1885_Max=654" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 654 bases. Perfect match Tm = 60.0 C, although only 17 nt are required to match. The actual sequence of the primer is cggatggcat gacagtaaga gaattatg." CDS complement(5666..5686) /label="HGTI-1886_Max=647" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 647 bases. Perfect match Tm = 60.2 C, although only 17 nt are required to match. The actual sequence of the primer is ccttttttgc ggcattttgc c." CDS complement(5967..5986) /label="HGTI-1887_Max=206" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 206 bases. Perfect match Tm = 58.6 C, although only 17 nt are required to match. The actual sequence of the primer is aagctgtgac cgtctccggg ." CDS 1121..1142 /label="HGTI-1978_Max=" /note="MLV LTR sequencing Max recorded sequencing read = bases. Perfect match Tm = 60.3 C, although only 17 nt are required to match. The actual sequence of the primer is CGTCAGCGGG GTCTTTCATT TG." CDS complement(1563..1590) /label="HGTI-1979_Max=0" /note="MLV LTR sequencing Max recorded sequencing read = 0 bases. Perfect match Tm = 61.3 C, although only 17 nt are required to match. The actual sequence of the primer is TCAGACAGAG ACAACACAGA ACGATGCT." CDS complement(1420..1444) /label="HGTI-1980_Max=0" /note="MLV LTR sequencing Max recorded sequencing read = 0 bases. Perfect match Tm = 61.8 C, although only 17 nt are required to match. The actual sequence of the primer is GGGGGTGCAC CAAAGAGTCC TAAAC." CDS complement(3711..3734) /label="HGTI-2082_Max=0" /note="Reverse sequencing primer for pUC. Purpose of the oligo: Sequencing Max recorded sequencing read = 0 bases. Perfect match Tm = 56.6 C, although only 17 nt are required to match. The actual sequence of the primer is AGCGGATAAC AATTTCACAC AGGA." ^^ AAGCTTGCATGCCTGCAGGTCGACTCTAGGCACATAAAGAAAAACATAACTAACCAAGCTGCAGCCGAGACAGTGAAAAGAACCGTTAAAACGGTTTGTTTTAAATAAACTGAATTATTTAGAGTCATTTCTTTGGTAGGAAAGTACATTGGCACGTAAAGGAGCCCAAAGCAATCTGTGGAAAGCCCAGGCTGGGAGCCCAGCAGTTTGCATCCCCTCCTGGCGTGTACCTAAGGGTTTCTTAATTGTGTGGTTTCTAAATCTTCCAGAGGGTTTGTCTCATTCACTTCCACTTCGGTGCACAATACTTGGACGCGGATTTACTGTCTTAGCATCTATCGGTGGCCCTTCGATTGAGGCTGAACCTGAGGCCCACTTCTTCAGCTTGTTAAGGAGAGCACAAGCACCAGAAGAGGCTGACCCGGCAGACCTGTGGGCATTTTTAACAAGGGCCTCCTGGGTCTGTGGGAGGCAGGCTTACATAAGGTGCAAATTAGAAATATAAATAATAAGCCCATATCAATTTGTCATCTTTTTTTAAGCTCAAGTTTTGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGTTGGAACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTTCTCAATAAACCCTCTTGCAGTTGCATCCGACTCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGTCTTTCATTTGGAGGTTCCACCGAGATTTGGAGACCCCTGCCCAGGGACCACCGACCCCCCCGCCGGGAGGTAAGCTGGCCAGCAACTTATCTGTGTCTGTCCGATTGTCTAGTGTCTATGACTGATTTTATGCGCCTGCGTCGGTACTAGTTAGCTAACTAGCTCTGTATCTGGCGGACCCGTGGTGGAACTGACGAGTTCGGAACACCCGGCCGCAACCCTGGGAGACGTCCCAGGGACTTCGGGGGCCGTTTTTGTGGCCCGACCTGAGTCCTAAAATCCCGATCGTTTAGGACTCTTTGGTGCACCCCCCTTAGAGGAGGGATATGTGGTTCTGGTAGGAGACGAGAACCTAAAACAGTTCCCGCCTCCGTCTGAATTTTTGCTTTCGGTTTGGGACCGAAGCCGCGCCGCGCGTCTTGTCTGCTGCAGCATCGTTCTGTGTTGTCTCTGTCTGACTGTGTTTCTGTATTTGTCTGAAAATATGGGCCCGGGCTAGACTGTTACCACTCCCTTAAGTTTGACCTTAGGTCACTGGAAAGATGTCGAGCGGATCGCTCACAACCAGTCGGTAGATGTCAAGAAGAGACGTTGGGTTACCTTCTGCTCTGCAGAATGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAACCGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGCCCGCATGGACACCCAGACCAGGTCCCCTACATCGTGACCTGGGAAGCCTTGGCTTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACACCCTAAGCCTCCGCCTCCTCTTCCTCCATCCGCCCCGTCTCTCCCCCTTGAACCTCCTCGTTCGACCCCGCCTCGATCCTCCCTTTATCCAGCCCTCACTCCTTCTCTAGGCGCCCCCATATGGCCATATGAGATCTTATATGGGGCACCCCCGCCCCTTGTAAACTTCCCTGACCCTGACATGACAAGAGTTACTAACAGCCCCTCTCTCCAAGCTCACTTACAGGCTCTCTACTTAGTCCAGCACGAAGTCTGGAGACCTCTGGCGGCAGCCTACCAAGAACAACTGGACCGACCGGTGGTACCTCACCCTTACCGAGTCGGCGACACAGTGTGGGTCCGCCGACACCAGACTAAGAACCTAGAACCTCGCTGGAAAGGACCTTACACAGTCCTGCTGACCACCCCCACCGCCCTCAAAGTAGACGGCATCGCAGCTTGGATACACGCCGCCCACGTGAAGGCTGCCGACCCCGGGGGTGGACCATCCTCTAGACTGCCATGGCGCGGATCCGGATTAGTCCAATTTGTTAAAGACAGGATATCAGTGGTCCAGGCTCTAGTTTTGACTCAACAATATCACCAGCTGAAGCCTATAGAGTACGAGCCATAGATAAAATAAAAGATTTTATTTAGTCTCCAGAAAAAGGGGGGAATGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGTTGGAACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTTCTCAATAAACCCTCTTGCAGTTGCATCCGACTCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGTCTTTCAGTTTCTCCCACCTACACAGGTCTCACTAACATTCCTGATGTGCCGCAGGGACTCCGTCAGCCCGGTTTTTGTTTATAATAAAATGCAAGAACAGTGTTCCCTTCAAGCCAGACTACATCCTGACTCTCGGCTTTATAAAAGAATGTTGAAGGGCTCTGTGGACTATCTGCCACACGACTTTTAAGATTTTTATGCCTCCTGGATGAGGGATTTAGTCAATCTATCCTCGTCTATTTTGCTGGCTTCTCCGTATTTTAAATTTCTAGTTTGCACTCCCTTCCTGAGAGCACGGCGATTGCAGAGTAGTTAATACTCTGAGGGCAGGCTTCTGTGAAAAGGTTGCCTGGGCTCAGTGTGAGATTTTGCCATAAAAAGGGGTCCTGCCCCTGTGTACAGACAGATCGGAATCTAGAGTGCATACTCAGAGTCCCCGCGGTTCCGGGGCTCTGATCTCAGGGCATCTTTGCCTAGAGATCCTCTACGCCGGACGCATCGTGGCCGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCC