LOCUS HRST 9909 bp DNA SYN 31-Mar-2003 DEFINITION HR'CMVegfpSinTrip with unique Cla I site. =============================== This ligation was reprocessed on 2/14/2002. The fragment histories are: Fragment #1: 1224 This fragment sequence was corrected on 2/14/2002 by correction record #187. Hypothesized error in all Trono Naldini vectors, in the gpt gene, which causes the gpt to be truncated. Restores reading frame. ============================ This sequence was corrected on 2/14/2002 using correction record #187, Hypothesized error in all Trono Naldini vectors, in the gpt gene, which causes the gpt to be truncated. Restores reading frame. TGGCGGTCTGTACCGGGTGCGTTAC replaced with TGGCGGTCTGGTACCGGGTGCGTTAC. This plasmid sequence was corrected on 3/18/2002, with correction record #136) From sequencing HRST clones, junction errors in making sequence file. From using a different linker, 3085 instead of 2553. Replacement of ATCGATGCTCGAGACCTAGAAAAACATGGA with ATCGATAGTCGAGACCTAGAAAAACATGGA. This plasmid sequence was corrected on 3/18/2002, with correction record #190) Difference between low and high copy plasmids. Many of our lab plasmids are high copy, yet the sequence file suggests that they are low copy. This correction could be applied to make the sequence properly represent the actual DNA sequence for a high copy plasmid. It was confirmed in the full plasmid sequencing of CMMP-MR1-1TcR, which has the pUC18 backbone. Replacement of GATACCAAATACTGTCCTTCTAGTGTAGCCGT with GATACCAAATACTGTTCTTCTAGTGTAGCCGT. =============================== This ligation was reprocessed on 5/9/2002. The fragment histories are: Fragment #1: 1224 This fragment sequence was corrected on 2/14/2002 by correction record #187. Hypothesized error in all Trono Naldini vectors, in the gpt gene, which causes the gpt to be truncated. Restores reading frame. ============================ =============================== This ligation was reprocessed on 5/28/2002. The fragment histories are: Fragment #1: 1224 This fragment sequence was corrected on 2/14/2002 by correction record #187. Hypothesized error in all Trono Naldini vectors, in the gpt gene, which causes the gpt to be truncated. Restores reading frame. This fragment sequence was corrected on 5/27/2002 by correction record #135. From sequencing HRST clones. Actually results from error in original sequence file. Broadening the tag for this sequence to include all HR-CMV-lacZ derived plasmids. This fragment sequence was corrected on 5/27/2002 by correction record #197. HRST CMV promoter sequence error. Restores perfect match to a genbank record. This fragment sequence was corrected on 5/27/2002 by correction record #198. There is not a functional Kpn I site at this location. Scheduling sequencing to learn actual sequence. Replacement sequence is original sequence from Naldini, which stops gpt orf. ============================ =============================== This ligation was reprocessed on 8/20/2002. The fragment histories are: Fragment #1: 1224 This fragment sequence was corrected on 2/14/2002 by correction record #187. Hypothesized error in all Trono Naldini vectors, in the gpt gene, which causes the gpt to be truncated. Restores reading frame. This fragment sequence was corrected on 5/27/2002 by correction record #135. From sequencing HRST clones. Actually results from error in original sequence file. Broadening the tag for this sequence to include all HR-CMV-lacZ derived plasmids. This fragment sequence was corrected on 5/27/2002 by correction record #197. HRST CMV promoter sequence error. Restores perfect match to a genbank record. This fragment sequence was corrected on 5/27/2002 by correction record #198. There is not a functional Kpn I site at this location. Scheduling sequencing to learn actual sequence. Replacement sequence is original sequence from Naldini, which stops gpt orf. This fragment sequence was corrected on 8/20/2002 by correction record #199. Another HRST CMV promoter sequence error. Restores match to other CMV sequence in our database and genbank. ============================ =============================== This ligation was reprocessed on 11/18/2002. The fragment histories are: Fragment #1: 1224 This fragment sequence was corrected on 2/14/2002 by correction record #187. Hypothesized error in all Trono Naldini vectors, in the gpt gene, which causes the gpt to be truncated. Restores reading frame. This fragment sequence was corrected on 5/27/2002 by correction record #135. From sequencing HRST clones. Actually results from error in original sequence file. Broadening the tag for this sequence to include all HR-CMV-lacZ derived plasmids. This fragment sequence was corrected on 5/27/2002 by correction record #197. HRST CMV promoter sequence error. Restores perfect match to a genbank record. This fragment sequence was corrected on 5/27/2002 by correction record #198. There is not a functional Kpn I site at this location. Scheduling sequencing to learn actual sequence. Replacement sequence is original sequence from Naldini, which stops gpt orf. This fragment sequence was corrected on 8/20/2002 by correction record #199. Another HRST CMV promoter sequence error. Restores match to other CMV sequence in our database and genbank. This fragment sequence was corrected on 11/18/2002 by correction record #211. Xcm I site error in all HRST backbones. ============================ SOURCE ORGANISM HIV REFERENCE 1 (bases 1 to 9909) AUTHORS Gray,JT, Mulligan,RC TITLE Direct Submission JOURNAL Submitted (31-Mar-2003) John T. Gray, Richard C. Mulligan Telephone: (617) 432-0045 E-mail: gray@hihg.med.harvard.edu Harvard Gene Therapy Initiative Vector Core Lab, Room 412 Harvard Institutes for Medicine 77 Avenue Louis Pasteur Boston, MA 02115 USA FEATURES Locations/Qualifiers plasmid 1..9909 /note="HRST, 9909 bp. Made via ligation #807." misc_feature 2893..2892 /note="HR'CMVeGFPSINTrip: #1224, Cla I(5')--Cla I(5') 9909 bp." BASE COUNT 2867 a 2155 c 2305 g 2582 t ORIGIN 1 GTGATTCCTC TGAATTATTT TCATTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA 61 ACTTAGTAGT TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG 121 ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC 181 AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA 241 GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG 301 GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC 361 GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG 421 GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA 481 GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA 541 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 601 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 661 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG 721 GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA 781 GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC 841 GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA 901 TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT 961 ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA 1021 TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA 1081 GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG 1141 ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC 1201 ACCGCACAGC AAGCGGCCGC TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG 1261 GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC 1321 CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT 1381 CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT 1441 ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT 1501 TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG 1561 AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC 1621 TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT 1681 GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC 1741 AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA 1801 ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT 1861 GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT 1921 TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC 1981 CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA 2041 GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCT CGACGGTATC GCCGAATTCA 2101 CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA 2161 GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA 2221 ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTGGGGC 2281 GATAAGCTTG GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 2341 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 2401 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 2461 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 2521 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 2581 TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT 2641 AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT 2701 TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC 2761 AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC 2821 GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGACTCT 2881 AGAGGATCCT ATCGATAGTC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 2941 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 3001 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 3061 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 3121 TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 3181 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 3241 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 3301 AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT 3361 TGCAAAGAAA TGAATATCAG AGAGTGAGAG GCCTTGACAT TATAATAGAT TTAGCAGGAA 3421 TTGAACTAGG AGTGGAGCAC ACAGGCAAAG CTGCAGAAGT ACTTGGAAGA AGCCACCAGA 3481 GATACTCACG ATTCTGCACA TACCTGGCTA ATCCCAGATC CTAAGGATTA CATTAAGTTT 3541 ACTAACATTT ATATAATGAT TTATAGTTTA AAGTATAAAC TTATCTAATT TACTATTCTG 3601 ACAGATATTA ATTAATCCTC AAATATCATA AGAGATGATT ACTATTATCC CCATTTAACA 3661 CAAGAGGAAA CTGAGAGGGA AAGATGTTGA AGTAATTTTC CCACAATTAC AGCATCCGTT 3721 AGTTACGACT CTATGATCTT CTGACACAAA TTCCATTTAC TCCTCACCCT ATGACTCAGT 3781 CGAATATATC AAAGTTATGG ACATTATGCT AAGTAACAAA TTACCCTTTT ATATAGTAAA 3841 TACTGAGTAG ATTGAGAGAA GAAATTGTTT GCAAACCTGA ATAGCTTCAA GAAGAAGAGA 3901 AGTGAGGATA AGAATAACAG TTGTCATTTA ACAAGTTTTA ACAAGTAACT TGGTTAGAAA 3961 GGGATTCAAA TGCATAAAGC AAGGGATAAA TTTTTCTGGC AACAAGACTA TACAATATAA 4021 CCTTAAATAT GACTTCAAAT AATTGTTGGA ACTTGATAAA ACTAATTAAA TATTATTGAA 4081 GATTATCAAT ATTATAAATG TAATTTACTT TTAAAAAGGG AACATAGAAA TGTGTATCAT 4141 TAGAGTAGAA AACAATCCTT ATTATCACAA TTTGTCAAAA CAAGTTTGTT ATTAACACAA 4201 GTAGAATACT GCATTCAATT AAGTTGACTG CAGATTTTGT GTTTTGTTAA AATTAGAAAG 4261 AGATAACAAC AATTTGAATT ATTGAAAGTA ACATGTAAAT AGTTCTACAT ACGTTCTTTT 4321 GACATCTTGT TCAATCATTG ATCGAAGTTC TTTATCTTGG AAGAATTTGT TCCAAAGACT 4381 CTGAAATAAG GAAAACAATC TATTATATAG TCTCACACCT TTGTTTTACT TTTAGTGATT 4441 TCAATTTAAT AATGTAAATG GTTAAAATTT ATTCTTCTCT GAGATCATTT CACATTGCAG 4501 ATAGAAAACC TGAGACTGGG GTAATTTTTA TTAAAATCTA ATTTAATCTC AGAAACACAT 4561 CTTTATTCTA ACATCAATTT TTCCAGTTTG ATATTATCAT ATAAAGTCAG CCTTCCTCAT 4621 CTGCAGGTTC CACAACAAAA ATCCAACCAA CTGTGGATCA AAAATATTGG GAAAAAATTA 4681 AAAATAGCAA TACAACAATA AAAAAATACA AATCAGAAAA ACAGCACAGT ATAACAACTT 4741 TATTTAGCAT TTACAATCTA TTAGGTATTA TAAGTAATCT AGAATTAATT CCGTGTATTC 4801 TATAGTGTCA CCTAAATCGT ATGTGTATGA TACATAAGGT TATGTATTAA TTGTAGCCGC 4861 GTTCTAACGA CAATATGTAC AAGCCTAATT GTGTAGCATC TGGCTTACTG AAGCAGACCC 4921 TATCATCTCT CTCGTAAACT GCCGTCAGAG TCGGTTTGGT TGGACGAACC TTCTGAGTTT 4981 CTGGTAACGC CGTCCCGCAC CCGGAAATGG TCAGCGAACC AATCAGCAGG GTCATCGCTA 5041 GCCAGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG 5101 CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA 5161 TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG 5221 CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC 5281 TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAATGGTG CACTCTCAGT 5341 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC 5401 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC 5461 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC 5521 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 5581 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 5641 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 5701 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 5761 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 5821 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 5881 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 5941 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 6001 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 6061 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 6121 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 6181 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 6241 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 6301 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 6361 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 6421 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 6481 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 6541 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 6601 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 6661 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 6721 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 6781 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 6841 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 6901 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 6961 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 7021 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 7081 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA 7141 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 7201 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 7261 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 7321 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 7381 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 7441 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 7501 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 7561 TGCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 7621 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 7681 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 7741 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG 7801 GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC 7861 AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT GGACACAAGA 7921 CAGGCTTGCG AGATATGTTT GAGAATACCA CTTTATCCCG CGTCAGGGAG AGGCAGTGCG 7981 TAAAAAGACG CGGACTCATG TGAAATACTG GTTTTTAGTG CGCCAGATCT CTATAATCTC 8041 GCGCAACCTA TTTTCCCCTC GAACACTTTT TAAGCCGTAG ATAAACAGGC TGGGACACTT 8101 CACATGAGCG AAAAATACAT CGTCACCTGG GACATGTTGC AGATCCATGC ACGTAAACTC 8161 GCAAGCCGAC TGATGCCTTC TGAACAATGG AAAGGCATTA TTGCCGTAAG CCGTGGCGGT 8221 CTGTACCGGG TGCGTTACTG GCGCGTGAAC TGGGTATTCG TCATGTCGAT ACCGTTTGTA 8281 TTTCCAGCTA CGATCACGAC AACCAGCGCG AGCTTAAAGT GCTGAAACGC GCAGAAGGCG 8341 ATGGCGAAGG CTTCATCGTT ATTGATGACC TGGTGGATAC CGGTGGTACT GCGGTTGCGA 8401 TTCGTGAAAT GTATCCAAAA GCGCACTTTG TCACCATCTT CGCAAAACCG GCTGGTCGTC 8461 CGCTGGTTGA TGACTATGTT GTTGATATCC CGCAAGATAC CTGGATTGAA CAGCCGTGGG 8521 ATATGGGCGT CGTATTCGTC CCGCCAATCT CCGGTCGCTA ATCTTTTCAA CGCCTGGCAC 8581 TGCCGGGCGT TGTTCTTTTT AACTTCAGGC GGGTTACAAT AGTTTCCAGT AAGTATTCTG 8641 GAGGCTGCAT CCATGACACA GGCAAACCTG AGCGAAACCC TGTTCAAACC CCGCTTTAAA 8701 CATCCTGAAA CCTCGACGCT AGTCCGCCGC TTTAATCACG GCGCACAACC GCCTGTGCAG 8761 TCGGCCCTTG ATGGTAAAAC CATCCCTCAC TGGTATCGCA TGATTAACCG TCTGATGTGG 8821 ATCTGGCGCG GCATTGACCC ACGCGAAATC CTCGACGTCC AGGCACGTAT TGTGATGAGC 8881 GATGCCGAAC GTACCGACGA TGATTTATAC GATACGGTGA TTGGCTACCG TGGCGGCAAC 8941 TGGATTTATG AGTGGGCCCC GGATCTTTGT GAAGGAACCT TACTTCTGTG GTGTGACATA 9001 ATTGGACAAA CTACCTACAG AGATTTAAAG CTCTAAGGTA AATATAAAAT TTTTAAGTGT 9061 ATAATGTGTT AAACTACTGA TTCTAATTGT TTGTGTATTT TAGATTCCAA CCTATGGAAC 9121 TGATGAATGG GAGCAGTGGT GGAATGCCTT TAATGAGGAA AACCTGTTTT GCTCAGAAGA 9181 AATGCCATCT AGTGATGATG AGGCTACTGC TGACTCTCAA CATTCTACTC CTCCAAAAAA 9241 GAAGAGAAAG GTAGAAGACC CCAAGGACTT TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA 9301 TGCTGTGTTT AGTAATAGAA CTCTTGCTTG CTTTGCTATT TACACCACAA AGGAAAAAGC 9361 TGCACTGCTA TACAAGAAAA TTATGGAAAA ATATTCTGTA ACCTTTATAA GTAGGCATAA 9421 CAGTTATAAT CATAACATAC TGTTTTTTCT TACTCCACAC AGGCATAGAG TGTCTGCTAT 9481 TAATAACTAT GCTCAAAAAT TGTGTACCTT TAGCTTTTTA ATTTGTAAAG GGGTTAATAA 9541 GGAATATTTG ATGTATAGTG CCTTGACTAG AGATCATAAT CAGCCATACC ACATTTGTAG 9601 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 9661 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 9721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 9781 AACTCATCAA TGTATCTTAT CATGTCTGGA TCAACTGGAT AACTCAAGCT AACCAAAATC 9841 ATCCCAAACT TCCCACCCCA TACCCTATTA CCACTGCCAA TTACCTGTGG TTTCATTTAC 9901 TCTAAACCT //