Plasmid Name = AAV-MCS Database Index Number = 1576 Created by: HIHG Description: AAV vector plasmid with MDM cassette and polylinker.=============================== This ligation was reprocessed on 10/1/2002. The fragment histories are: Fragment #1: 1147 Fragment #2: 1148 This fragment sequence was corrected on 9/30/2002 by correction record #201. AAV non-vector error, probably present in all AAV constructs. Merely deletes 3 bp prior to left ITR sequence. ============================ FEATURES Locations/Qualifiers plasmid 1..6147 /note="AAV-MCS, 6147 bp. Made via ligation #775." misc_feature 1019..1543 /note="AAV-nlsCre: #1147, SanD I(5')--Not I(5') 525 bp." misc_feature 1544..1018 /note="AAV-hOB: #1148, Not I(5')--SanD I(5') 5622 bp." misc_feature 1007..1482 /label="B-globin_intron" /note="Human B-globin 2nd intron, Rsa I deleted." polyA_site 1787..1811 /label="polyA_site" /note="Axxs added after these bases in globin message." misc_feature 2335..2359 /label="End_of_globin" /note="End of globin sequences in typical pMD constructs." CDS 4295..5152 /label="bla" /note="B-lactamase, confers ampicillin resistance, no Pst I." misc_feature 856..907 /label="CMV_TATA" /note="CMV TATA box sequences, from -52 to -1. Wild type, with Sac I site." misc_feature 5451..5494 /label="Low_copy" /note="Tag for low copy plasmid replication xxpBR322xx" promoter 237..907 /label="CMV" /note="CMV promoter. Transcription starts with next base. 5 prime end represents complete extent of the CMV promoter in MDM based plasmids, which corresponds to positions 471..1141 of hCMVie genbank record accession X03922." repeat_region 2449..2489 /label="A_AAV_ITR" /note="A section of AAV type 2 ITR." repeat_region 52..60 /label="B_AAV_ITR" /note="Stem of B stem loop from AAV ITR" repeat_region 2490..2498 /label="B_AAV_ITR" /note="Stem of B stem loop from AAV ITR" repeat_region 30..38 /label="C_AAV_ITR" /note="Stem of C stem loop from AAV ITR" repeat_region 2512..2520 /label="C_AAV_ITR" /note="Stem of C stem loop from AAV ITR" repeat_region 114..133 /label="D_AAV_ITR" /note="D region of AAV ITR" misc_feature 908..978 /label="CMV_5xxUTR" /note="5xx untranslated region from CMV immediate early promoter. The 3 prime end corresponds to the full extent of CMV sequences in the MDM constructs." misc_feature 1600..2359 /label="beta_globin_pA" /note="From 3rd exon of human beta globin, contains polyA sequences commonly used in HGTI vector lab constructs." misc_feature 76..99 /label="RepBS" /note="Rep binding site" promoter 324..907 /label="CMV" /note="CMV promoter. Transcription starts with next base. 5 prime end starts at Spe I site only. The CMV region in this construct may extend further upstream." repeat_region complement(73..113) /label="A_AAV_ITR" /note="A section of AAV type 2 ITR." repeat_region complement(64..72) /label="B_AAV_ITR" /note="Stem of B stem loop from AAV ITR" repeat_region complement(2502..2510) /label="B_AAV_ITR" /note="Stem of B stem loop from AAV ITR" repeat_region complement(42..50) /label="C_AAV_ITR" /note="Stem of C stem loop from AAV ITR" repeat_region complement(2524..2532) /label="C_AAV_ITR" /note="Stem of C stem loop from AAV ITR" repeat_region complement(2429..2448) /label="D_AAV_ITR" /note="D region of AAV ITR" misc_feature complement(2463..2486) /label="RepBS" /note="Rep binding site" misc_feature complement(5458..5489) /label="Correction-190" /note="Difference between low and high copy plasmids. Many of our lab plasmids are high copy, yet the sequence file suggests that they are low copy. This correction could be applied to make the sequence properly represent the actual DNA sequence for a high copy plasmid. It was confirmed in the full plasmid sequencing of CMMP-MR1-1TcR, which has the pUC18 backbone. This sequence has been found to be erroneous for some plasmids in our laboratory. It would be prudent to sequence the DNA to confirm the accuracy of your records. The sequence specified by this feature was actually found to be: ACGGCTACAC TAGAAGAACA GTATTTGGTA TC. " CDS 920..943 /label="HGTI-761_Max=" /note="PCR or sequencing primer located in CMV sequence just after 5xx end of RNA transcript. Max recorded sequencing read = bases. Perfect match Tm = 63.9 C, although only 17 nt are required to match. The actual sequence of the primer is GAGACGCCAT CCACGCTGTT TTGA." CDS complement(890..931) /label="HGTI-1192_Max=" /note="linker from SacI of CMV bridging into BglII of HIV R. Max recorded sequencing read = bases. Perfect match Tm = 74.8 C, although only 17 nt are required to match. The actual sequence of the primer is gatcTGGTCT AACCAGAGAG ACCCcggttc actaaacgAG CT." CDS 845..866 /label="HGTI-1204_Max=311" /note="Sequencing primers for pHJ1 Max recorded sequencing read = 311 bases. Perfect match Tm = 62.5 C, although only 17 nt are required to match. The actual sequence of the primer is GCAAATGGGC GGTAGGCGTG TA." CDS 840..880 /label="HGTI-1220_Max=162" /note="? Max recorded sequencing read = 162 bases. Perfect match Tm = 76.1 C, although only 17 nt are required to match. The actual sequence of the primer is gcgcactagt catatggtag gcgtgtacgg tgggaggtct a." CDS 1452..1472 /label="HGTI-1278_Max=0" /note="Jeng Shin designed sequencing primer @ 5xx end of MDM2b polylinker facing towards gene. xx723 is longer, higher Tm versionxx. Max recorded sequencing read = 0 bases. Perfect match Tm = 40.3 C, although only 17 nt are required to match. The actual sequence of the primer is CATGTTCATA CCTCTTATCT T." CDS complement(1646..1663) /label="HGTI-1279_Max=" /note="Jeng Shin designed sequencing primer @ 3xx end of MDM2b polylinker facing towards gene.New incarnation is 3149, reordered 1xx29xx01. Max recorded sequencing read = bases. Perfect match Tm = 54.2 C, although only 17 nt are required to match. The actual sequence of the primer is gccagggcat tagccaca." CDS 2581..2606 /label="HGTI-1288_Max=" /note="sequencing primers for AAV backbone Max recorded sequencing read = bases. Perfect match Tm = 72.8 C, although only 17 nt are required to match. The actual sequence of the primer is CCGCACCGAT CGCCCTTCCC AACAGT." CDS complement(2760..2789) /label="HGTI-1289_Max=" /note="sequencing primers for AAV backbone Max recorded sequencing read = bases. Perfect match Tm = 67 C, although only 17 nt are required to match. The actual sequence of the primer is GCCACCGAGT AAAAGAGTCT GTCCATCACG ." CDS 3809..3836 /label="HGTI-1290_Max=" /note="sequencing primers for AAV backbone Max recorded sequencing read = bases. Perfect match Tm = 60.5 C, although only 17 nt are required to match. The actual sequence of the primer is TTTTGCTAAT TCTTTGCCTT GCCTGTAT." CDS complement(3962..3985) /label="HGTI-1291_Max=577" /note="sequencing primers for AAV backbone Max recorded sequencing read = 577 bases. Perfect match Tm = 71.4 C, although only 17 nt are required to match. The actual sequence of the primer is GTCAGCGGGT GTTGGCGGGT GTCG." CDS complement(3926..3956) /label="HGTI-1662_Max=680" /note="Series of primers for construction of 4 variations of Adenovirus helper constructs, with potential for deletions in Psi. Max recorded sequencing read = 680 bases. Perfect match Tm = 67.2 C, although only 17 nt are required to match. The actual sequence of the primer is GGCTTAACTA TGCGGCATCA GAGCAGATTG T." CDS 1437..1471 /label="HGTI-1732_Max=783" /note="New 5xx sequencing primer for B-globin constructs. Same position as JSL-70, but longer with a Tm at 66C. Max recorded sequencing read = 783 bases. Perfect match Tm = 65.5 C, although only 17 nt are required to match. The actual sequence of the primer is GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT." CDS 379..406 /label="HGTI-1832_Max=638" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 638 bases. Perfect match Tm = 58.7 C, although only 17 nt are required to match. The actual sequence of the primer is agttccgcgt tacataactt acggtaaa." CDS 979..1003 /label="HGTI-1834_Max=595" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 595 bases. Perfect match Tm = 58.7 C, although only 17 nt are required to match. The actual sequence of the primer is cctcgaagct gatcctgaga acttc." CDS 1279..1310 /label="HGTI-1835_Max=639" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 639 bases. Perfect match Tm = 58.2 C, although only 17 nt are required to match. The actual sequence of the primer is gttaaggcaa tagcaatatt tctgcatata aa." CDS 1769..1797 /label="HGTI-1843_Max=379" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 379 bases. Perfect match Tm = 59.8 C, although only 17 nt are required to match. The actual sequence of the primer is agcatctgga ttctgcctaa taaaaaaca." CDS 2070..2103 /label="HGTI-1844_Max=643" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 643 bases. Perfect match Tm = 59.1 C, although only 17 nt are required to match. The actual sequence of the primer is ttttacatta cttattgttt tagctgtcct catg." CDS complement(5919..5938) /label="HGTI-1850_Max=589" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 589 bases. Perfect match Tm = 59.7 C, although only 17 nt are required to match. The actual sequence of the primer is taaaaaggcc gcgttgctgg ." CDS complement(5620..5638) /label="HGTI-1851_Max=601" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 601 bases. Perfect match Tm = 60.1 C, although only 17 nt are required to match. The actual sequence of the primer is cgaccgctgc gccttatcc." CDS complement(5309..5338) /label="HGTI-1852_Max=372" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 372 bases. Perfect match Tm = 58.8 C, although only 17 nt are required to match. The actual sequence of the primer is aaaaggatct caagaagatc ctttgatctt ." CDS complement(5017..5038) /label="HGTI-1853_Max=0" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 0 bases. Perfect match Tm = 61.0 C, although only 17 nt are required to match. The actual sequence of the primer is ccccagtgct gcaatgatac cg." CDS complement(4718..4738) /label="HGTI-1854_Max=650" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 650 bases. Perfect match Tm = 58.4 C, although only 17 nt are required to match. The actual sequence of the primer is agcggttagc tccttcggtc c." CDS complement(4415..4438) /label="HGTI-1855_Max=661" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 661 bases. Perfect match Tm = 58.0 C, although only 17 nt are required to match. The actual sequence of the primer is atccagttcg atgtaaccca ctcg." CDS complement(4108..4138) /label="HGTI-1856_Max=711" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 711 bases. Perfect match Tm = 58.3 C, although only 17 nt are required to match. The actual sequence of the primer is gacattaacc tataaaaata ggcgtatcac g." CDS complement(464..488) /label="HGTI-1863_Max=672" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 672 bases. Perfect match Tm = 59.1 C, although only 17 nt are required to match. The actual sequence of the primer is ccctattggc gttactatgg gaaca." CDS complement(764..788) /label="HGTI-1864_Max=485" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 485 bases. Perfect match Tm = 58.0 C, although only 17 nt are required to match. The actual sequence of the primer is aaacaaactc ccattgacgt caatg." CDS complement(1061..1088) /label="HGTI-1865_Max=636" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 636 bases. Perfect match Tm = 59.3 C, although only 17 nt are required to match. The actual sequence of the primer is tgttacttct ccccttccta tgacatga." CDS complement(1363..1388) /label="HGTI-1866_Max=639" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 639 bases. Perfect match Tm = 59.3 C, although only 17 nt are required to match. The actual sequence of the primer is gcagaatggt agctggattg tagctg." CDS complement(1854..1878) /label="HGTI-1874_Max=692" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 692 bases. Perfect match Tm = 58.1 C, although only 17 nt are required to match. The actual sequence of the primer is ttaaatgcac tgacctccca cattc." CDS complement(2149..2179) /label="HGTI-1875_Max=610" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 610 bases. Perfect match Tm = 59.5 C, although only 17 nt are required to match. The actual sequence of the primer is tatctctaag caagagaact gagtggagtc a." CDS 5829..5854 /label="HGTI-1881_Max=511" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 511 bases. Perfect match Tm = 59 C, although only 17 nt are required to match. The actual sequence of the primer is atctttatag tcctgtcggg tttcgc." CDS 5529..5556 /label="HGTI-1882_Max=565" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 565 bases. Perfect match Tm = 60.5 C, although only 17 nt are required to match. The actual sequence of the primer is catacctcgc tctgctaatc ctgttacc." CDS 5229..5257 /label="HGTI-1883_Max=564" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 564 bases. Perfect match Tm = 58.2 C, although only 17 nt are required to match. The actual sequence of the primer is tgaagatcct ttttgataat ctcatgacc." CDS 4929..4953 /label="HGTI-1884_Max=593" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 593 bases. Perfect match Tm = 59.6 C, although only 17 nt are required to match. The actual sequence of the primer is ataaagttgc aggaccactt ctgcg." CDS 4629..4656 /label="HGTI-1885_Max=654" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 654 bases. Perfect match Tm = 60.0 C, although only 17 nt are required to match. The actual sequence of the primer is cggatggcat gacagtaaga gaattatg." CDS 4329..4349 /label="HGTI-1886_Max=647" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 647 bases. Perfect match Tm = 60.2 C, although only 17 nt are required to match. The actual sequence of the primer is ccttttttgc ggcattttgc c." CDS 4029..4048 /label="HGTI-1887_Max=206" /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD Max recorded sequencing read = 206 bases. Perfect match Tm = 58.6 C, although only 17 nt are required to match. The actual sequence of the primer is aagctgtgac cgtctccggg ." CDS 789..821 /label="HGTI-2021_Max=707" /note="sequencing pHD from 3xx end Max recorded sequencing read = 707 bases. Perfect match Tm = 71.5 C, although only 17 nt are required to match. The actual sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa tgt." CDS complement(1180..1209) /label="HGTI-2022_Max=610" /note="sequencing primer in globin intron facing left. Max recorded sequencing read = 610 bases. Perfect match Tm = 53.9 C, although only 17 nt are required to match. The actual sequence of the primer is gaaagaaaga gattagggaa agtattagaa ." CDS 1454..1481 /label="HGTI-2144_Max=0" /note="sFlt clone Add-musFlt-mIg: sequencing ORF Max recorded sequencing read = 0 bases. Perfect match Tm = 59.3 C, although only 17 nt are required to match. The actual sequence of the primer is TGTTCATACC TCTTATCTTC CTCCCACA." ^^ CAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGCGGCCTCGGCCTCTGCATAAATAAAAAAAATTAGTCAGCCATGAGCTTGGCCCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCCCTCGAAGCTGATCCTGAGAACTTCAGGGTGAGTCTATGGGACCCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGAAGGGGAGAAGTAACAGGGTACACATATTGACCAAATCAGGGTAATTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCCGCGGGCGGCCGCTCTAGAGGATCCAAGCTTATCGATACCGTCGACCTCGAGGGCCCAGATCTAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGCAATGATGTATTTAAATTATTTCTGAATATTTTACTAAAAAGGGAATGTGGGAGGTCAGTGCATTTAAAACATAAAGAAATGAAGAGCTAGTTCAAACCTTGGGAAAATACACTATATCTTAAACTCCATGAAAGAAGGTGAGGCTGCAAACAGCTAATGCACATTGGCAACAGCCCCTGATGCCTATGCCTTATTCATCCCTCAGAAAAGGATTCAAGTAGAGGCTTGATTTGGAGGTTAAAGTTTTGCTATGCTGTATTTTACATTACTTATTGTTTTAGCTGTCCTCATGAATGTCTTTTCACTACCCATTTGCTTATCCTGCATCTCTCAGCCTTGACTCCACTCAGTTCTCTTGCTTAGAGATACCACCTTTCCCCTGAAGTGTTCCTTCCATGTTTTACGGCGAGATGGTTTCTCCTCGCCTGGCCACTCAGCCTTAGTTGTCTCTGTTGTCTTATAGAGGTCTACTTGAAGAAGGAAAAACAGGGGGCATGGTTTGACTGTCCTGTGAGCCCTTCTTCCCTGCCTCCCCCACTCACAGTGACCCGGAATCCCTCGACATCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGATTCTGTTGCAATGGCTGGCGGTAATATTGTTCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGTTATTACTAATCAAAGAAGTATTGCGACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACTCGGTGGCCTCACTGATTATAAAAACACTTCTCAGGATTCTGGCGTACCGTTCCTGTCTAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCTAACGAGGAAAGCACGTTATACGTGCTCGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCGGGGTACATATGATTGACATGCTAGTTTTACGATTACCGTTCATCGATTCTCTTGTTTGCTCCAGACTCTCAGGCAATGACCTGATAGCCTTTGTAGAGACCTCTCAAAAATAGCTACCCTCTCCGGCATGAATTTATCAGCTAGAACGGTTGAATATCATATTGATGGTGATTTGACTGTCTCCGGCCTTTCTCACCCGTTTGAATCTTTACCTACACATTACTCAGGCATTGCATTTAAAATATATGAGGGTTCTAAAAATTTTTATCCTTGCGTTGAAATAAAGGCTTCTCCCGCAAAAGTATTACAGGGTCATAATGTTTTTGGTACAACCGATTTAGCTTTATGCTCTGAGGCTTTATTGCTTAATTTTGCTAATTCTTTGCCTTGCCTGTATGATTTATTGGATGTTGGAATGCCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATG