LOCUS AAV-MCS 6147 bp DNA SYN 31-Mar-2003 DEFINITION AAV vector plasmid with MDM cassette and polylinker.=============================== This ligation was reprocessed on 10/1/2002. The fragment histories are: Fragment #1: 1147 Fragment #2: 1148 This fragment sequence was corrected on 9/30/2002 by correction record #201. AAV non-vector error, probably present in all AAV constructs. Merely deletes 3 bp prior to left ITR sequence. ============================ SOURCE ORGANISM AAV REFERENCE 1 (bases 1 to 6147) AUTHORS Gray,JT, Mulligan,RC TITLE Direct Submission JOURNAL Submitted (31-Mar-2003) John T. Gray, Richard C. Mulligan Telephone: (617) 432-0045 E-mail: gray@hihg.med.harvard.edu Harvard Gene Therapy Initiative Vector Core Lab, Room 412 Harvard Institutes for Medicine 77 Avenue Louis Pasteur Boston, MA 02115 USA FEATURES Locations/Qualifiers plasmid 1..6147 /note="AAV-MCS, 6147 bp. Made via ligation #775." misc_feature 1019..1543 /note="AAV-nlsCre: #1147, SanD I(5')--Not I(5') 525 bp." misc_feature 1544..1018 /note="AAV-hOB: #1148, Not I(5')--SanD I(5') 5622 bp." BASE COUNT 1499 a 1502 c 1409 g 1737 t ORIGIN 1 CAGCTGCGCG CTCGCTCGCT CACTGAGGCC GCCCGGGCAA AGCCCGGGCG TCGGGCGACC 61 TTTGGTCGCC CGGCCTCAGT GAGCGAGCGA GCGCGCAGAG AGGGAGTGGC CAACTCCATC 121 ACTAGGGGTT CCTTGTAGTT AATGATTAAC CCGCCATGCT ACTTATCTAC GTAGCCATGC 181 TCTAGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGAG CTTGGCCCAT 241 TGCATACGTT GTATCCATAT CATAATATGT ACATTTATAT TGGCTCATGT CCAACATTAC 301 CGCCATGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG 361 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT 421 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC 481 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG 541 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT 601 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 661 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 721 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 781 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 841 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG 901 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC 961 GGGACCGATC CAGCCTCCCC TCGAAGCTGA TCCTGAGAAC TTCAGGGTGA GTCTATGGGA 1021 CCCTTGATGT TTTCTTTCCC CTTCTTTTCT ATGGTTAAGT TCATGTCATA GGAAGGGGAG 1081 AAGTAACAGG GTACACATAT TGACCAAATC AGGGTAATTT TGCATTTGTA ATTTTAAAAA 1141 ATGCTTTCTT CTTTTAATAT ACTTTTTTGT TTATCTTATT TCTAATACTT TCCCTAATCT 1201 CTTTCTTTCA GGGCAATAAT GATACAATGT ATCATGCCTC TTTGCACCAT TCTAAAGAAT 1261 AACAGTGATA ATTTCTGGGT TAAGGCAATA GCAATATTTC TGCATATAAA TATTTCTGCA 1321 TATAAATTGT AACTGATGTA AGAGGTTTCA TATTGCTAAT AGCAGCTACA ATCCAGCTAC 1381 CATTCTGCTT TTATTTTATG GTTGGGATAA GGCTGGATTA TTCTGAGTCC AAGCTAGGCC 1441 CTTTTGCTAA TCATGTTCAT ACCTCTTATC TTCCTCCCAC AGCTCCTGGG CAACGTGCTG 1501 GTCTGTGTGC TGGCCCATCA CTTTGGCAAA GAATTCCGCG GGCGGCCGCT CTAGAGGATC 1561 CAAGCTTATC GATACCGTCG ACCTCGAGGG CCCAGATCTA ATTCACCCCA CCAGTGCAGG 1621 CTGCCTATCA GAAAGTGGTG GCTGGTGTGG CTAATGCCCT GGCCCACAAG TATCACTAAG 1681 CTCGCTTTCT TGCTGTCCAA TTTCTATTAA AGGTTCCTTT GTTCCCTAAG TCCAACTACT 1741 AAACTGGGGG ATATTATGAA GGGCCTTGAG CATCTGGATT CTGCCTAATA AAAAACATTT 1801 ATTTTCATTG CAATGATGTA TTTAAATTAT TTCTGAATAT TTTACTAAAA AGGGAATGTG 1861 GGAGGTCAGT GCATTTAAAA CATAAAGAAA TGAAGAGCTA GTTCAAACCT TGGGAAAATA 1921 CACTATATCT TAAACTCCAT GAAAGAAGGT GAGGCTGCAA ACAGCTAATG CACATTGGCA 1981 ACAGCCCCTG ATGCCTATGC CTTATTCATC CCTCAGAAAA GGATTCAAGT AGAGGCTTGA 2041 TTTGGAGGTT AAAGTTTTGC TATGCTGTAT TTTACATTAC TTATTGTTTT AGCTGTCCTC 2101 ATGAATGTCT TTTCACTACC CATTTGCTTA TCCTGCATCT CTCAGCCTTG ACTCCACTCA 2161 GTTCTCTTGC TTAGAGATAC CACCTTTCCC CTGAAGTGTT CCTTCCATGT TTTACGGCGA 2221 GATGGTTTCT CCTCGCCTGG CCACTCAGCC TTAGTTGTCT CTGTTGTCTT ATAGAGGTCT 2281 ACTTGAAGAA GGAAAAACAG GGGGCATGGT TTGACTGTCC TGTGAGCCCT TCTTCCCTGC 2341 CTCCCCCACT CACAGTGACC CGGAATCCCT CGACATCTAG AGCATGGCTA CGTAGATAAG 2401 TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC 2461 TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC 2521 TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCCAGC TGGCGTAATA GCGAAGAGGC 2581 CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GATTCTGTTG 2641 CAATGGCTGG CGGTAATATT GTTCTGGATA TTACCAGCAA GGCCGATAGT TTGAGTTCTT 2701 CTACTCAGGC AAGTGATGTT ATTACTAATC AAAGAAGTAT TGCGACAACG GTTAATTTGC 2761 GTGATGGACA GACTCTTTTA CTCGGTGGCC TCACTGATTA TAAAAACACT TCTCAGGATT 2821 CTGGCGTACC GTTCCTGTCT AAAATCCCTT TAATCGGCCT CCTGTTTAGC TCCCGCTCTG 2881 ATTCTAACGA GGAAAGCACG TTATACGTGC TCGTCAAAGC AACCATAGTA CGCGCCCTGT 2941 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 3001 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 3061 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 3121 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 3181 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 3241 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 3301 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 3361 AACAAAATAT TAACGTTTAC AATTTAAATA TTTGCTTATA CAATCTTCCT GTTTTTGGGG 3421 CTTTTCTGAT TATCAACCGG GGTACATATG ATTGACATGC TAGTTTTACG ATTACCGTTC 3481 ATCGATTCTC TTGTTTGCTC CAGACTCTCA GGCAATGACC TGATAGCCTT TGTAGAGACC 3541 TCTCAAAAAT AGCTACCCTC TCCGGCATGA ATTTATCAGC TAGAACGGTT GAATATCATA 3601 TTGATGGTGA TTTGACTGTC TCCGGCCTTT CTCACCCGTT TGAATCTTTA CCTACACATT 3661 ACTCAGGCAT TGCATTTAAA ATATATGAGG GTTCTAAAAA TTTTTATCCT TGCGTTGAAA 3721 TAAAGGCTTC TCCCGCAAAA GTATTACAGG GTCATAATGT TTTTGGTACA ACCGATTTAG 3781 CTTTATGCTC TGAGGCTTTA TTGCTTAATT TTGCTAATTC TTTGCCTTGC CTGTATGATT 3841 TATTGGATGT TGGAATGCCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC 3901 ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC 3961 CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC 4021 TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC 4081 ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT 4141 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC 4201 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 4261 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC 4321 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT 4381 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT 4441 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC 4501 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT 4561 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA 4621 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA 4681 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT 4741 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA 4801 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG 4861 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT 4921 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT 4981 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC 5041 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA 5101 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC 5161 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG 5221 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC 5281 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 5341 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 5401 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 5461 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 5521 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 5581 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 5641 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 5701 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 5761 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 5821 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 5881 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 5941 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC 6001 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 6061 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT 6121 CCCCGCGCGT TGGCCGATTC ATTAATG //