The Harvard Gene Therapy Initiative Vector Construction Service

HGTI can do plasmid subcloning as a service for the construction of a new virus. We are currently limiting this service to inserting genes as fragments of plasmids provided by the customer into specific HGTI plasmid vectors, which are also available to laboratories for their own cloning projects. For detailed information on our vectors, please see the Vector Data Resource (text-based) or its graphical interface.     We have used these vectors to construct various reporter gene containing plasmids, and these plasmids are available from our facility to use in virus production. To see a partial list of these plasmids, click here. Other plasmids may also be available; please direct inquiries to Dr. Jeng-Shin Lee.     We provide the following vectors:

Vector Name	Virus Type	Expression Cassette	Additional genes	Capacity
AAV-MCS	Adeno-associated virus	CMV-b-globin	none	~2,000 bp
Ad-Cla	E1/E3 deleted adenovirus	CMV-b-globin	none	~5,000 bp
Ad-BGFP-Cla	E1/E3 deleted adenovirus	Bidirectional CMV-b-globin	GFP	~3,250 bp
MMP	MPSV/MLV derived retrovirus	MMP	none	~6,250 bp
MMP-iresGFP	MPSV/MLV derived retrovirus	MMP	GFP	~4,900 bp
MMP-iresGFPneo	MPSV/MLV derived retrovirus	MMP	GFPneo fusion protein	~4,100 bp
HRST	3' Enhancer deleted HIV derived lentivirus	CMV-lenti	none	~6,800 bp